Tentative Workshop Schedules

2010 Workshop Schedule

Day 1 (August 23th)

Location: Genome and Biomedical Science Facility, Room 2202; UC Davis

Check-in/Welcome
8:00am  - 8:30am        -       Check-in, Coffee, Tea 
8:30am  - 9:00am        -       Welcome, Introduction, Overview
Development of a TILLING Population + Resource
9:00am  - 9:30am	-       Objective, Strategy, Guidelines 
9:30am  - 10:00am	-       Case Study: Wheat
10:00am - 10:30am	-       Polyploidy and Definition of Targets 
-Coffee Break-
10:45am - 11:15am 	-	Case Study: Tomato 
11:15am - 12:00pm	-	DNA Preps and Automation
Lunch @ Scrubs Cafe
 1:30pm - 2:15pm	-	DNA Standardization and Arraying
 2:15pm - 2:45pm	-	Pooling Strategies
-Break-
Illumina Sequencing Library Construction
3:00pm - 3:30pm         -       Overview
3:30pm - 4:45pm         -       Protocol
4:45pm - 5:00pm         -       Q+A
Day 2 (August 24th)

Location: Genome and Biomedical Science Facility Room 2202, UC Davis

Tour of TILLING Facilities
 9:00am - 9:15am	-	Meet in GBSF 4503 Common Room 
 9:15am - 10:00am	-	TILLING Facilities Tour
-Coffee Break-
Illumina Technology and TILLING Run Setup
10:15am - 10:30am	-       Illumina sequencing technology (and competitors) 
10:30am - 10:45am	-       From complete library to sequence file 
10:45am - 11:00am	-       Run parameters: read length, pair, cost, reference, filtering; Problems and troubleshooting TILLING runs
Background Bioinformatics
11:00am - 12:00pm	-       Barcodes and spikes; Computer resources; Alignment and assembly program
Lunch @ Scrubs Cafe
TILLING Bioinformatics Pipeline
 1:30pm - 2:00pm	-	Target information, possible problems 
 2:00pm - 3:00pm	-	Sequence input and filtering; BWA, parsing, and preliminary analysis; Mutation discovery
Mutation Report
 3:30pm - 4:15pm	-	Threshold setting strategies;    Verification; Pool deconvolution  
 4:15pm - 4:45pm	-	Screening via CEL1
 4:45pm - 5:00pm	-	Q+A

END

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