2010 Workshop Schedule
Day 1 (August 23th)
Location: Genome and Biomedical Science Facility, Room 2202; UC Davis
Check-in/Welcome
8:00am - 8:30am - Check-in, Coffee, Tea 8:30am - 9:00am - Welcome, Introduction, Overview
Development of a TILLING Population + Resource
9:00am - 9:30am - Objective, Strategy, Guidelines 9:30am - 10:00am - Case Study: Wheat 10:00am - 10:30am - Polyploidy and Definition of Targets
-Coffee Break-
10:45am - 11:15am - Case Study: Tomato 11:15am - 12:00pm - DNA Preps and Automation
Lunch @ Scrubs Cafe
1:30pm - 2:15pm - DNA Standardization and Arraying 2:15pm - 2:45pm - Pooling Strategies
-Break-
Illumina Sequencing Library Construction
3:00pm - 3:30pm - Overview 3:30pm - 4:45pm - Protocol 4:45pm - 5:00pm - Q+A
Day 2 (August 24th)
Location: Genome and Biomedical Science Facility Room 2202, UC Davis
Tour of TILLING Facilities
9:00am - 9:15am - Meet in GBSF 4503 Common Room 9:15am - 10:00am - TILLING Facilities Tour
-Coffee Break-
Illumina Technology and TILLING Run Setup
10:15am - 10:30am - Illumina sequencing technology (and competitors) 10:30am - 10:45am - From complete library to sequence file 10:45am - 11:00am - Run parameters: read length, pair, cost, reference, filtering; Problems and troubleshooting TILLING runs
Background Bioinformatics
11:00am - 12:00pm - Barcodes and spikes; Computer resources; Alignment and assembly program
Lunch @ Scrubs Cafe
TILLING Bioinformatics Pipeline
1:30pm - 2:00pm - Target information, possible problems 2:00pm - 3:00pm - Sequence input and filtering; BWA, parsing, and preliminary analysis; Mutation discovery
Mutation Report
3:30pm - 4:15pm - Threshold setting strategies; Verification; Pool deconvolution 4:15pm - 4:45pm - Screening via CEL1 4:45pm - 5:00pm - Q+A
