2011 Workshop Schedule
Day 1 (August 22nd)
Location: Meet at GBSF 4503 Common Room
Tour of TILLING Facilities and Tomato TILLING populations
Check-in/Welcome
8:00am - 8:15am - Check-in, Coffee, Tea 8:15am - 8:30am - Welcome & Introductions
Tour of the Fields
8:40am - 10:00am - Fields 10:00am - 10:30am - Break
Tour of the Lab
10:30am - 11:30am - TILLING Core Lab & Genome Center Sequencing Facilities
Lunch
Dinner
Day 2 (August 23rd)
Location: Genome and Biomedical Science Facility, Room 2202; UC Davis
Check-in/Welcome
8:00am - 8:15am - Check-in, Coffee, Tea 8:15am - 8:45am - Welcome, Introduction, Overview
Development of a TILLING Population + Resource
8:45am - 9:00am - Objective, Strategy, Guidelines 9:00am - 9:30am - Case Study: Wheat 9:30am - 10:00am - Case Study: Tomato
Coffee Break
10:30am - 11:00am - DNA Standardization and Pooling Strategies 11:00am - 11:30pm - Illumina and Barcoding 11:30am - 12:00pm - Q & A
Lunch
Bioinformatics
Illumina Technology and Tilling Run Setup
1:30pm - 1:40pm - Intro to Bioinformatics 1:40pm - 2:00pm - Picking Targets
Tilling Bioinformatics Pipeline
2:00pm - 2:50pm - Data Analysis: Preprocessing Stage 2:50pm - 3:05pm - Break 3:05pm - 3:30pm - Data Analysis: Mutation Detection Stage using CAMBa
Mutation Report
3:30pm - 3:55pm - Results and Verification 3:55pm - 4:10pm - Q & A
New Developments
4:10pm - 4:30pm - Exome Capture 4:30pm - 5:00pm - Resequencing at Restriction Enzyme Sites