This page summarizes the methods available from the Comai lab
TILLING and High throughput sequencing
- Illumina library construction with amplicons, or any input DNA
- Bioinformatics pipeline for mutation discovery
Custom barcoding for Illumina and Illumina barcoded reads pipeline
- Barcoded Data Preparation Tools
- Custom barcoded Illumina adapters
Genotyping and Mutation Discovery
- a Genotyping by Sequencing method
- BWA-DoAll, a package for batch library processing and alignment
- Tools for Mpileup generation and parsing
- MAPS: a Mutation Detection and Genotyping tool
Exome capture: genotyping and mutation discovery
Discover CNV in genome (a Matt Porter and Korf lab collaboration)
Funding
This page and the connected tools are funded by:
- NSF Plant Genome grant DBI-0733857 (Functional Genomics of Polyploids)
- NSF Plant Genome grant DBI-0924025. Heterosis Challenge Grant (HCG):The Regulatory Disruption Hypothesis for Heterosis
- NSF Plant Genome grant DBI-0822383 (“TILLING by Sequencing”)
- National Institutes of Health R01 GM076103-01A1 (Dosage dependent regulation in hybridization) to LC.