Point Mutations in Centromeric Histone Induce Post-zygotic Incompatibility and Uniparental Inheritance In a paper published in Plos Genetics the Britt Lab (Sundaram Kuppu et al.) and the Comai lab report a simple non-transgenic approach to the production of haploid inducers. Any of multiple changes in the conserved region of centromeric histone H3 is sufficient to yield aContinue reading
A System for Dosage-Based Functional Genomics in Poplar In a paper published in the Plant Cell Isabelle Henry and collaborators from the Groover lab report the characterization of a mutant population of poplar produced by crossing Populus deltoides with irradiated pollen of P. nigra. The resulting F1 population of ~500 interspecific hybrids (soon to grow to ~800) providesContinue reading
Catastrophic chromosomal restructuring during genome elimination in plants In a paper in eLife published in May 2105 Han Tan and colleagues report that when Arabidopsis with weakened centromeres is crossed to the wild type, i.e. a plant with normal centromeres, the resulting embryos undergo chromothripsis, the cut-and-reassembly process leading to highly rearranged chromosomes. Because weakened centromeres can occur naturally,Continue reading
Naturally Occurring Differences in CENH3 Affect Chromosome Segregation in Zygotic Mitosis of Hybrids Genome elimination mediated by the chimeric “GFP-tailswap” CENH3 is a promising tool for the production of haploids. But, what is the significance of natural variation in CENH3? Shamoni Maheshwari et al. describe in PLoS Genetics (2015) how wide variation in CENH3 isContinue reading
Perturbation of Parentally Biased Gene Expression during Interspecific Hybridization Parental gene imprinting has been postulated to play a major role in postzygotic incompatibility. What happens to imprinted genes when two different species are mated? Diana Burkart-Waco et al. describe in PLoS One how paternally expressed genes (PEG) are frequently misregulated during interspecific hybridization.